Which of the following statements correctly identifies type II restriction endonucleases?
A)They work on both DNA and RNA.
B)They recognize a palindromic sequence and cut just before the palindromic sequence.
C)They degrade DNA by subsequently removing bases from each end.
D)They cut DNA only at sites in specific nucleotide sequences with a 2-fold axis of symmetry.

Question

Quizplus · Accepted Answer

Type II restriction endonucleases cut DNA only at sites in specific nucleotide sequences with a 2-fold axis of symmetry. They recognize the same sequence on both strands of the DNA double helix, which is usually a palindrome, meaning it reads the same way on both strands when read in opposite directions. The enzyme then cuts at a specific position within the recognition sequence, usually between the base pairs of the symmetry axis.

Which of the Following Statements Correctly Identifies Type II Restriction