Which of the following is NOT necessary for restriction endonuclease mapping of DNA molecules?
A)determining the 5'- and/or 3'-ends of the molecule
B)protease digestion of fragments
C)hydrolysis with 2 or more endonucleases
D)knowing where each endonuclease hydrolyzes

Question

Quizplus · Accepted Answer

Protease digestion of fragments is not necessary for restriction endonuclease mapping, as this process involves enzymes that cut DNA at specific sequences, not proteins. Proteases would digest proteins, not DNA.

Which of the Following Is NOT Necessary for Restriction Endonuclease