Which of the following is NOT correct for cDNA libraries?
A)Reverse transcriptase synthesizes a DNA strand complement of the mRNA templates.
B)mRNA templates are isolated using oligo (dA)-cellulose chromatography.
C)Linkers are added and the cDNA is cloned into suitable vectors.
D)The cDNA are copies from mRNA templates.

Question

Quizplus · Accepted Answer

cDNA libraries are usually constructed from mRNA molecules obtained from the cells or tissues of interest. The mRNA is isolated using poly(A) tail-specific oligonucleotide probes, not oligo(dA)-cellulose chromatography. The mRNA is then used as a template to produce a complementary DNA (cDNA) copy using the enzyme reverse transcriptase. The resulting cDNA is then cloned into a suitable vector, often after the addition of linkers. Therefore, option B is not correct because it suggests the use of a different method for mRNA isolation than the one commonly used for cDNA library construction.

Which of the Following Is NOT Correct for CDNA Libraries