To express a eukaryotic protein in E.coli,the eukaryotic cDNA must be cloned in an expression vector.What does the expression vector need to contain?
A)promoter and ribosomal-binding site
B)promoter and transition start site
C)ribosomal-binding site and hybridization complex
D)introns and exons

Question

Quizplus · Accepted Answer

The expression vector must contain a promoter to drive the transcription of the cDNA and a ribosomal-binding site to facilitate translation of the protein product. The other choices (B, C, D) are not relevant for eukaryotic protein expression in E.coli.

To Express a Eukaryotic Protein in E