What information is critical to the success of PCR itself?
A)The DNA sequence of the ends of the DNA to be amplified must be known.
B)The complete DNA sequence of the DNA to be amplified must be known.
C)The sequence of restriction enzyme recognition sites in the DNA to be amplified must be known.
D)The sequence of restriction enzyme recognition sites in the DNA to be amplified and in the plasmid where the amplified DNA fragment will be cloned must be known.

Question

Quizplus · Accepted Answer

The DNA sequence of the ends of the DNA to be amplified must be known in order to design primers specific for amplification of the desired fragment. PCR requires specific primers that anneal to the DNA sequence flanking the region of interest, so if the DNA sequence of the ends of the DNA to be amplified is not known, it is not possible to design appropriate primers. Therefore, this information is critical to the success of PCR itself.

What Information Is Critical to the Success of PCR Itself