Imagine that you've prepared a Southern blot and probed it using a single probe.You observe eight different bands after autoradiography,with one band being very dark and the other seven being significantly lighter.What is suggested by these results?
A)The gene recognized by the probe has been cut into eight different fragments by the restriction enzyme.
B)The probe has been cut into eight different fragments by the restriction enzyme.
C)There are eight similar genes in the genome.
D)The DNA did not transfer from the gel to the nylon filter.
E)The DNA remained double stranded on the blot.

Question

Quizplus · Accepted Answer

The presence of eight bands suggests that there are eight similar sequences in the genome that the probe is binding to, with one being more abundant or having a stronger affinity, resulting in a darker band.

Imagine That You've Prepared a Southern Blot and Probed It