If mRNAs could be ligated and replicated within plasmids, what enzyme commonly used in recombinant DNA technology would no longer be needed?
A) restriction enzymes
B) DNA polymerase
C) RNA polymerase
D) reverse transcriptase
E) Taq polymerase

Question

Quizplus · Accepted Answer

Reverse transcriptase is used to convert RNA into DNA, which is a necessary step before the DNA can be inserted into plasmids if working directly with mRNA. If mRNAs could be directly ligated and replicated within plasmids, this step, and thus the enzyme, would be unnecessary.

If MRNAs Could Be Ligated and Replicated Within Plasmids, What