What is a primary difference between polymerase chain reaction (PCR) and traditional cloning procedures such as those used to clone the human growth hormone gene?
A) PCR and traditional cloning make use of different types of bacteria.
B) PCR and traditional cloning make use of different types of vectors.
C) PCR uses plasmid vectors, whereas traditional cloning uses bacteria.
D) PCR eliminates the need for restriction enzymes, vectors, and cells.
E) PCR is more time-consuming, but the purity of the obtained DNA clone is much higher than in traditional cloning.

Question

Quizplus · Accepted Answer

PCR eliminates the need for restriction enzymes, vectors, and cells, distinguishing it from traditional cloning methods which require these components to insert DNA fragments into host cells for replication.

What Is a Primary Difference Between Polymerase Chain Reaction (PCR)