In a single polymerase chain reaction (PCR) cycle consisting of 15 seconds at 94°C, 30 seconds at 50°C, and 1 minute at 72°C, what is happening in the step run at 50°C?
A) The DNA to be amplified is being denatured.
B) Primers are being denatured.
C) DNA polymerase is extending new DNA from the primers.
D) Primers are annealing to the DNA to be amplified.
E) DNA polymerase is being inactivated.

Question

Quizplus · Accepted Answer

During the step run at 50°C, primers are annealing (binding) to the single-stranded DNA template. This step is crucial for the PCR reaction as it provides a template for the DNA polymerase to attach and start extension of new DNA strands.

In a Single Polymerase Chain Reaction (PCR) Cycle Consisting of 15