Which of the following most correctly describes the whole-genome shotgun technique for sequencing a genome?
A) genetic mapping followed immediately by sequencing
B) physical mapping followed immediately by sequencing
C) cloning large genome fragments into very large vectors such as YACs, followed by sequencing
D) cloning fragments from many copies of an entire chromosome, sequencing the fragments, and then ordering the sequences
E) cloning the whole genome directly, from one end to the other

Question

Quizplus · Accepted Answer

The whole-genome shotgun technique involves cloning many fragments from multiple copies of an entire chromosome, sequencing those fragments, and then reassembling the sequences in the correct order to reconstruct the entire genome. This approach bypasses the need for physical or genetic mapping, as the clones are randomly selected from throughout the genome.

Which of the Following Most Correctly Describes the Whole-Genome Shotgun