Induction of sporulation by Bacillus sp.can be accomplished with

Question

Quizplus · Accepted Answer

To induce sporulation, organisms can be grown on triple sugar iron agar (TSI), urea agar, or nutrient agar containing 5 mg/L of manganese sulfate. On Gram-stained smears, spores appear clear because they do not retain the crystal violet or safranin. However, spores will stain with specific dyes such as malachite green, which is forced into the spore using heat; the vegetative cell is then counterstained with safranin.

Induction of Sporulation by Bacillus Sp