Performance of the "factor requirement test" for Haemophilus involves

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Traditional identification criteria includes the requirement for X and/or V factors for growth. To establish X and V factor requirements, disks impregnated with each factor are placed on unsupplemented media, usually Mueller-Hinton agar or trypticase soy agar, that has been inoculated with a light suspension of the organism. After overnight incubation at 35° C in ambient air, the plate is examined for growth around each disk. Many X factor-requiring organisms are able to carry over enough factor from the primary medium to give False-negative results (i.e., growth occurs at such a distance from the X disk as to Falsely indicate that the organism does not require the X factor).

Performance of the "Factor Requirement Test" for Haemophilus Involves