To increase the sensitivity of direct Gram stain examination of body fluid specimens, the specimens usually are

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To increase the sensitivity of direct Gram stain examination of body fluid specimens, especially cerebrospinal fluid (CSF), the specimens usually are centrifuged (2000 rpm for 10 minutes) and the smear is prepared from the resulting pellet. This concentration step can increase the sensitivity of direct microscopic examination by five- to tenfold.

To Increase the Sensitivity of Direct Gram Stain Examination of Body