The permanent stained smear part of the O&P (ova and parasite) examination

Question

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The permanent stained smear is designed to facilitate identification of intestinal protozoa. Several staining methods are available; the two most common methods are the Wheatley modification of the Gomori tissue trichrome and the iron-hematoxylin stains. This part of the O&P examination is critical for the confirmation of suspicious objects seen in the wet examination and identification of protozoa that might not have been seen in the wet preparation. The permanent stained smear is the most important procedure performed for the identification of intestinal protozoan infections; the permanent stained smears are examined using oil immersion objectives (´600 for screening, ´1000 for final review of =300 oil immersion fields).

The Permanent Stained Smear Part of the O&P (Ova and Parasite)