Cytomegalovirus can be detected in clinical specimens using

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Cytomegalovirus can be detected in clinical specimens using conventional cell culture, shell vial assay, antigenemia immunoassay, or molecular methods. CMV produces cytopathic effects (CPE) in diploid fibroblast cells in 3 to 28 days, averaging 7 days. Shell vial for CMV has a sensitivity equivalent to conventional cell culture but takes only 16 hours to complete. The antigenemia immunoassay uses monoclonal antibody in an indirect immunoperoxidase or indirect immunofluorescent stain to detect CMV protein in peripheral blood leukocytes. The antigenemia assay requires only 3 to 5 hours to complete and includes separation of leukocytes by sedimentation, counting of leukocytes, smear preparation with a standard density of cells, and staining and counting of infected (fluorescing) cells.

Cytomegalovirus Can Be Detected in Clinical Specimens Using