Indirect immunofluorescent FA testing involves

Question

Quizplus · Accepted Answer

The indirect immunofluorescent procedure is a two-step test in which unlabeled antiviral antibody is added to the slide first, followed by a labeled (FITC) antiglobulin that binds to the first-step antibody bound to virus in the specimen. Increased sensitivity of the indirect test results from signal amplification that occurs with the addition of the second antibody. Signal amplification decreases specificity by increasing nonspecific background fluorescence. Indirect immunofluorescence should be used when lower quantities of virus are suspected, such as for detection of respiratory viruses in specimens from adult patients.

Indirect Immunofluorescent FA Testing Involves