Direct immunofluorescent FA testing involves

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Direct immunofluorescent testing involves use of a labeled antiviral antibody; the label is usually fluorescein isothiocyanate (FITC), which is layered over specimens suspected of containing homologous virus. Direct immunofluorescence is generally more rapid and specific than indirect immunofluorescence, but less sensitive. Direct immunofluorescence is best suited to situations in which large quantities of virus are suspected or high-quality, concentrated monoclonal antibodies are used, such as for the detection of RSV in patient specimens or the identification of viruses growing in cell culture.

Direct Immunofluorescent FA Testing Involves