Influenza A and B viruses can be detected in clinical specimens using

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Influenza A and B viruses are detected by using conventional cell culture, shell vial culture, membrane EIA (enzyme immunoassay), direct staining of respiratory tract secretions using FA methods, and PCR. PCR is superior to other methods. When cell culture is performed, primary monkey kidney cells are superior to other cell lines. Median time to detection, using hemadsorption at day 2 or 3, is 3 days. Fluorescent antibody staining is used for confirmation and typing of isolates as A or B. Nearly all influenza viruses are detected after 1 week of incubation.

Influenza a and B Viruses Can Be Detected in Clinical