Ultraviolet (UV)light can be used to estimate protein solution concentrations because
A)phenylalanine absorbs at 260 nm.
B)all the amino acids absorb UV light.
C)aromatic amino acids absorb at 280 nm.
D)tryptophan and tyrosine absorb at 280 nm.
E)All of the above.

Question

Quizplus · Accepted Answer

Only tryptophan and tyrosine absorb at 280 nm, which is the wavelength commonly used for estimating protein concentrations using UV spectroscopy. Phenylalanine absorption at 260 nm can interfere with accurate measurements, and not all amino acids absorb UV light.

Ultraviolet (UV)light Can Be Used to Estimate Protein Solution Concentrations