Concentrations of some proteins cannot be estimated by UV spectrophotometry because they are
A)low in glycine and valine.
B)low in tryptophan and tyrosine.
C)low in protein.
D)high in aromatic amino acids.

Question

Quizplus · Accepted Answer

UV spectrophotometry relies on the presence of aromatic amino acids (tryptophan and tyrosine) to estimate protein concentration. Proteins lacking sufficient amounts of these amino acids cannot be accurately quantified using this method.

Concentrations of Some Proteins Cannot Be Estimated by UV Spectrophotometry