Which technique is used less for protein purification and more for the determination of molecular weights?
A)Affinity chromatography.
B)SDS-PAGE.
C)Gel filtration.
D)Ion exchange chromatography.

Question

Quizplus · Accepted Answer

SDS-PAGE is used primarily for determining the molecular weights of proteins based on their migration rates through a gel under denaturing conditions. While it can be used for purification, it is not as efficient or specific as other techniques such as affinity chromatography or ion exchange chromatography. Gel filtration can also be used for purification, but it separates proteins based on size rather than charge or binding specificity.

Which Technique Is Used Less for Protein Purification and More