Which technique is most sensitive and accurate for the determination of a protein's molecular weight?
A)SDS-PAGE.
B)Gel filtration chromatography.
C)Mass spectrometry.
D)X-ray crystallography.
E)Osmotic pressure.

Question

Quizplus · Accepted Answer

Mass spectrometry is the most sensitive and accurate technique for determining a protein's molecular weight. It uses ionization and separation techniques to measure the mass-to-charge ratio of molecules and can provide accurate molecular weight information for large biomolecules such as proteins. SDS-PAGE and gel filtration chromatography can provide molecular weight estimates but are less precise than mass spectrometry. X-ray crystallography is a valuable technique for determining the structure of proteins but does not provide direct molecular weight information. Osmotic pressure can be used to determine relative molecular weights but is not as precise as mass spectrometry.

Which Technique Is Most Sensitive and Accurate for the Determination