Which might be a method used for distinguishing among several mechanistic possibilities in a multi-substrate reaction?
A)Measure Km in the presence of one substrate at a time.
B)Measure the rate with respect to one substrate while varying the concentration of another substrate.
C)Measure the rate while adding inactivators for all but one substrate.
D)Any of the above.

Question

Quizplus · Accepted Answer

Measuring the rate with respect to one substrate while varying the concentration of another substrate can help distinguish between different kinetic mechanisms by observing how the presence of one substrate affects the reaction rate of another.

Which Might Be a Method Used for Distinguishing Among Several