What method did Frederick Sanger use to elucidate the structure of insulin?
A)X-ray crystallography
B)bioinformatics
C)analysis of amino acid sequence of small fragments
D)NMR spectroscopy
E)high-speed centrifugation

Question

Quizplus · Accepted Answer

Frederick Sanger used the method of analyzing the amino acid sequence of small fragments to elucidate the structure of insulin. This involved breaking down and purifying insulin into smaller fragments, determining their amino acid sequence, and then assembling the fragments to determine the full structure of the protein. This method, known as protein sequencing, was the primary technique used to determine the amino acid sequences of proteins before the advent of DNA sequencing.

What Method Did Frederick Sanger Use to Elucidate the Structure