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Yeast is capable of synthesizing the amino acids necessary for survival and can grow on minimal media or enriched media easily. In both conditions, the colonies have a cream colour. The production of adenine is regulated by two genes, ADE1 and ADE2. If either of these genes is defective, adenine cannot be synthesized and the colony will look red (as a result of accumulation of precursors) . A common undergraduate lab study is to irradiate yeast cells with ultra violet light from 2-5 sec up to 1-5 minutes, and see what will happen.
-If, after irradiating and growing cultures in minimal media, you see no red cultures, what can you conclude?
A) No mutation occurred.
B) Mutations may have occurred but not in the adenine pathway.
C) All the cultures were mutated.
D) The mutants died because of a lack of adenine in the media.
E) You didn't leave the cultures under UV long enough.
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