A genetic study conducted over multiple generations found that the frequency of genotypes in the first generation were,AA: 0.36,Aa: 0.48,aa: 0.16.In the second generation,genotype frequencies shifted to AA: 0.16,Aa: 0.48,aa: 0.36.According to Hardy-Weinberg equilibrium,we can assume:

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The shift in genotype frequencies between the first and second generation indicates that some form of evolutionary force has been acting on the population. If the population is assumed to be in Hardy-Weinberg equilibrium, then the only possible evolutionary force that could be causing the shift is nonrandom mating. However, the fact that gene frequencies have shifted suggests that another force, such as gene flow, could also be playing a role. Therefore, the best choice is D, which includes both nonrandom mating and gene flow as possible factors contributing to the shift in genotype frequencies.

A Genetic Study Conducted Over Multiple Generations Found That the Frequency