After Reading the Paragraph Below, Answer the Questions That Follow

After reading the paragraph below, answer the questions that follow.
Studies of the endomembrane system often involve the use of a protein that can emit a green fluorescence (glow) . A researcher wants to make a video of cell behavior, so she initially tags the outer nuclear envelope of a cell with the fluorescent tag and records for several hours. Later, she sees that the tag is part of a secretory vesicle.
-The fluorescent tag likely did not become incorporated into which of the following organelles?

A) lysosome
B) Golgi
C) microtubules
D) rough ER