What makes eukaryotic transcripts easier to isolate than transcripts in bacteria?
A) Eukaryotic transcripts are not methylated but their genes are often methylated.
B) Larger transcript size in eukaryotes enables easy size-selection methods.
C) mRNA is polyadenylated in eukaryotes.
D) Transcripts are the most abundant RNAs in eukaryotes.

Question

Quizplus · Accepted Answer

mRNA in eukaryotic cells is polyadenylated, meaning they have a poly(A) tail at the 3' end that can be used for isolation by binding to oligo(dT) beads. Bacteria do not have polyadenylated mRNA, which makes it more difficult to isolate. A, B, and D are not correct answers as they do not accurately reflect the differences between eukaryotic and bacterial transcripts.

What Makes Eukaryotic Transcripts Easier to Isolate Than Transcripts in Bacteria