Some proteins overexpressed at high levels resulting in the formation of inclusion bodies can abolish the goal of producing large quantities of active protein.What could be done to minimize this issue?
A) Codon optimize the gene.
B) Decrease the number of biobricks in the vector.
C) Simultaneously produce intracellular chaperonins.
D) Switch to an expression host with a larger intracellular volume.

Question

Quizplus · Accepted Answer

Simultaneously producing intracellular chaperonins can help proteins fold correctly, reducing the formation of inclusion bodies and increasing the yield of active protein.

Some Proteins Overexpressed at High Levels Resulting in the Formation