A sample demonstrates a DAT profile that is positive with polyspecific AHG, negative with anti-IgG, and positive with anti-C3.If the Donath-Landsteiner test was also positive, what should the CLS do?

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Quizplus · Accepted Answer

The positive DAT profile with a negative anti-IgG and positive anti-C3, along with a positive Donath-Landsteiner test, suggest the possibility of paroxysmal cold hemoglobinuria (PCH). Conferencing with the supervisor is necessary to confirm the suspicion and proceed with appropriate testing and management. A cold agglutinin test would not provide any further information in this case. An elution and antibody panel or a serum sample for antibody screen may also be considered, but confirming or ruling out PCH would be the priority.

A Sample Demonstrates a DAT Profile That Is Positive with Polyspecific