Assume that a plasmid (circular)is 3200 base pairs in length and has restriction sites at the following locations: 400,700,1400,2600.Give the expected sizes of the restriction fragments following complete digestion.
A) 400, 800, 1000 (2 of these)
B) 400, 1200, 1600
C) 300, 700, 2200
D) 700, 400, 1400, 2600
E) 300, 700, 1000, 1200

Question

Quizplus · Accepted Answer

The plasmid is cut at 400, 700, 1400, and 2600 base pairs. The fragments will be: 400-700 (300 bp), 700-1400 (700 bp), 1400-2600 (1200 bp), and 2600-400 (1000 bp).

Assume That a Plasmid (Circular)is 3200 Base Pairs in Length