Some vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region.Which term is given to this advantageous arrangement of restriction sites?
A) palindrome
B) consensus sequence
C) multiple cloning site
D) β-galactosidase
E) complementation

Question

Quizplus · Accepted Answer

The advantageous arrangement of restriction sites in vectors such as pUC18 and others of the pUC series is called the multiple cloning site (MCS) or polylinker. The MCS consists of a short stretch of DNA that contains multiple unique restriction enzyme recognition sequences, allowing for easy and efficient cloning of DNA fragments. Palindromes are sequences that read the same backward as forward, which may be present in some restriction enzyme recognition sequences but is not specific to the clustered arrangement in pUC vectors. Consensus sequence refers to the most commonly observed nucleotide at each position of a DNA sequence, which is not relevant in this context. β-galactosidase and complementation are terms related to gene expression and complementation analysis, respectively, and are not directly relevant to the arrangement of restriction sites in pUC vectors.

Some Vectors Such as PUC18 and Others of the PUC