Some restriction enzymes cleave DNA in such a manner as to produce blunt ends.Most often ligation of blunt end fragments is enhanced by the use of the enzyme terminal deoxynucleotidyl transferase.Speculate on the function of deoxynucleotidyl transferase in terms of using blunt end fragments in cloning.

Question

Quizplus · Accepted Answer

The Answer is Terminal deoxynucleotidyl transferase extends single-stranded ends by the addition of nucleotide tails.If complementary tails are added,the fragments can hybridize and the recombinant molecules can be ligated.

Some Restriction Enzymes Cleave DNA in Such a Manner as to Produce