If you started the PCR technique with two fragments of double stranded DNA,calculate how many double stranded DNA fragments you would you have after four cycles.

Question

Quizplus · Accepted Answer

Each cycle of PCR doubles the amount of DNA, so after 4 cycles, there will be 2^4 = 16 times the original amount of DNA. Since each double-stranded fragment becomes two double-stranded fragments after one cycle, the initial two fragments will become 32 fragments after four cycles. Therefore, the best choice is C) 32.

If You Started the PCR Technique with Two Fragments of Double