The definition of the microscopic working distance is the distance from the:

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The working distance is the distance from the bottom of the objective to the material being studied.The greater the magnifying power of a lens,the smaller is the focal length and thus the working distance.This becomes very important when the microscope is being used,because the working distance is very short for the 40× (4 mm)and 100× (1.8 mm)objectives.For this reason,correct focusing habits are necessary to prevent damaging the objectives against the slide on the stage.

The Definition of the Microscopic Working Distance Is the Distance