Fluorescent objects (natural,stained,or labeled)can be seen with a fluorescent microscope because they absorb the ____ light and emit light of yellow or ____ wavelength.

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Certain objects have the ability to fluoresce,which means they absorb light of certain very short (ultraviolet)wavelengths and emit light of longer (visible)wavelengths.In fluorescence microscopy with transmitted light and a compound microscope,the darkfield condenser is preceded by a special exciter filter that allows only shorter wavelength blue light to pass and cross on the specimen plane.If the specimen contains an object that fluoresces (either naturally or because of staining or labeling with certain fluorescent dyes),it will absorb the blue light and emit light of a longer yellow or green wavelength.

Fluorescent Objects (Natural,stained,or Labeled)can Be Seen with a Fluorescent Microscope