Which of the following is a sterilization procedure that uses moist heat and how is this method effective in killing bacterial spores?
A) Filtration; filters all microbes larger than the filter pore size
B) Boiling; kills all microorganisms and spores at 100° C (boiling)
C) Hot-air chamber; kills all microorganisms and bacterial spores at 171° C for at least 1 hour
D) Autoclaving; kills all microorganisms and bacterial spores at 121° C for 15 minutes at 15 lb above atmospheric pressure
Correct Answer:
Verified
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