Detection of a product in real-time PCR depends on
A) the separation of the reporter from the quencher.
B) the reporter and quencher remaining in close proximity to each other.
C) the reporter binding to the quencher.
D) Taq polymerase destroying the reporter.

Question

Quizplus · Accepted Answer

Real-time PCR relies on the separation of the fluorescent reporter from the quencher. During amplification, the probe hybridizes to the target DNA and Taq polymerase cleaves the probe, separating the reporter molecule from the quencher molecule. This allows the reporter to emit fluorescence, which can be detected in real-time as the reaction progresses. Thus, choice A is the correct answer. Choices B and C are incorrect, as they describe the state of the probe before Taq polymerase cleavage. Choice D is incorrect, because Taq polymerase is not involved in destroying the reporter molecule.

Detection of a Product in Real-Time PCR Depends on