You are performing a mobility shift assay with a protein complex that is composed of proteins A,B,C,& D,each of which can bind to the DNA of interest.You run the gel with the lanes as follows:
Lane 1: Protein A + DNA
Lane 2: Proteins A + B + DNA
Lane 3: Proteins A + B + C + DNA
Lane 4: Proteins A + B + C + D + DNA
In which lane will the DNA run closest to the top of the gel?
A) Lane 4
B) Lane 3
C) Lane 2
D) Lane 1

Question

Quizplus · Accepted Answer

The DNA will run closest to the top of the gel in Lane 4 because it has the most proteins bound to it, resulting in the largest complex and therefore the slowest migration through the gel.

You Are Performing a Mobility Shift Assay with a Protein