Pyrosequencing is an example of a high-throughput,next-generation sequencing technology.What is a crucial detail of the procedure?
A) including the enzyme luciferase
B) mixing the solution of four nucleotides together in even amounts
C) removing nucleotides from the target DNA one at a time
D) using large wells to accommodate multiple beads

Question

Quizplus · Accepted Answer

Pyrosequencing involves the use of the enzyme luciferase, which is important in detecting the release of pyrophosphate during the incorporation of nucleotides into the DNA strand.

Pyrosequencing Is an Example of a High-Throughput,next-Generation Sequencing Technology