After a metagenomics researcher collects a mixed sample of DNA,what next step allows this DNA to be studied?
A) The DNA fragments are each ligated into a cloning vector to create a DNA library.
B) The pieces of DNA are ligated together into one complete "genome" so they can be sequenced.
C) The species responsible for each DNA are identified and individually cultured.
D) DNA is reconstructed into chromosomes by using the human genome as a scaffold.

Question

Quizplus · Accepted Answer

The next step after collecting a mixed sample of DNA in metagenomics is to create a library of the DNA fragments. This is done by ligating the DNA fragments into a cloning vector, which allows the fragments to be replicated and studied further. Option A is the correct choice as it provides the correct next step in the process.

Option B is incorrect because it assumes that the DNA fragments can be ligated together to create a complete genome. This is not typically possible in metagenomics due to the complexity of the sample.

Option C is also incorrect because it would be time-consuming and difficult to individually culture all of the species present in the mixed sample.

Option D is incorrect because using the human genome as a scaffold would not be appropriate for studying DNA from a mixed sample that likely includes a diverse range of species.

After a Metagenomics Researcher Collects a Mixed Sample of DNA,what