What is the purpose of the cyanogen bromide (CNBr) in the manufacture of insulin and somatostatin by recombinant bacteria?
A)Allows for the uptake of the plasmid
B)Enhances the activity of the restriction enzymes
C)Creates sticky ends for integration into the plasmid
D)Cleaves the protein from the b-galactosidase protein

Question

Quizplus · Accepted Answer

Cyanogen bromide (CNBr) is used to cleave proteins at methionine residues, which is useful for separating the desired protein (like insulin or somatostatin) from fusion proteins such as β-galactosidase in recombinant protein production.

What Is the Purpose of the Cyanogen Bromide (CNBr) in the Manufacture