Using the Neo^R/Tk system of introducing gene replacements into mice (as described in the text), which of the following would identify those mouse cells that were the result of homologous recombination? A)Neo^R only B)Tk only C)Neo^R and Tk D)Neither Neo^R or Tk

Question

Quizplus · Accepted Answer

In the Neo^R/Tk system, cells that have undergone homologous recombination will be resistant to the antibiotic G418 due to the presence of the Neo^R gene, but will not have the Tk gene, which would make them sensitive to ganciclovir. Thus, Neo^R only indicates successful homologous recombination.

Using the NeoR/Tk System of Introducing Gene Replacements into Mice

Using the Neo^R/Tk System of Introducing Gene Replacements into Mice