GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The primer used for replication in vitro is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively?
A)Left, left
B)Right, left
C)Left, right
D)Right, right

Question

Quizplus · Accepted Answer

The primer used for replication in vitro is CCCGGTAAGCTT, which should bind to the template strand in the 3' to 5' direction to synthesize the new strand in the 5' to 3' direction. Therefore, the 5' end of the template strand would be on the left, and the 5' end of the primer would be on the right. Choice C reflects this understanding.