The phenomenon that allows a quencher dye to reduce the fluorescence of the reporter dye in real-time PCR techniques is called:

Question

Quizplus · Accepted Answer

The phenomenon described is referred to as fluorescence resonance energy transfer (FRET), which occurs when a quencher dye is in close proximity to a fluorescent or reporter dye. The quencher dye absorbs the energy emitted by the reporter dye, which reduces the fluorescence signal detected. This technique is commonly used in real-time PCR to quantify the amount of target DNA amplified during the reaction. Choices A, C, and D are not relevant to this phenomenon.

The Phenomenon That Allows a Quencher Dye to Reduce the Fluorescence