How is bacterial DNA cross-linked to putative binding proteins for chromatin immunoprecipitation (ChIP)?

Question

Quizplus · Accepted Answer

Bacterial DNA is cross-linked to putative binding proteins using formaldehyde. This binds proteins to DNA and prepares the samples for chromatin immunoprecipitation (ChIP). Heat, reduction with dithiothreitol, and reduction with F1F1F1S1F1F1F10-mercaptoethanol are not commonly used for bacterial ChIP assays. UV light can be used for DNA cross-linking in some cases, but it is less commonly used due to the potential for DNA damage.

How Is Bacterial DNA Cross-Linked to Putative Binding Proteins for Chromatin