Which is true for the relationship between quencher concentration and fluorescent intensity?
A)As quencher concentration increases,fluorescent intensity increases.
B)As quencher concentration decreases,fluorescent intensity decreases.
C)As quencher concentration increases,fluorescent intensity decreases.
D)As quencher concentration increases,fluorescent intensity remains constant.
E)As quencher concentration decreases,fluorescent concentration remains constant.

Question

Quizplus · Accepted Answer

The relationship between quencher concentration and fluorescent intensity is that as the quencher concentration increases, the fluorescent intensity decreases. This is because the quencher molecule absorbs the excitation energy from the fluorescent molecule, reducing the amount of fluorescent light emitted. Therefore, when the concentration of quencher molecules is higher, they are more likely to collide with the fluorescent molecules, causing a decrease in fluorescent intensity.

Which Is True for the Relationship Between Quencher Concentration and Fluorescent