Which statements are true for molecular exclusion chromatography?
I Molecular exclusion chromatography separates on the basis of size.
II Small molecules that freely pass through the stationary phase,K_av = 0.
III Small molecules can fit in the pores of the stationary phase and effectively pass through a larger volume,and elute last.
IV The pores in the stationary phase are too small for large molecules to pass through,so large molecules elute first.
V Molecular exclusion chromatography with a hydrophilic stationary phase and an aqueous solvent is called gel permeation chromatography.

Question

Quizplus · Accepted Answer

Molecular exclusion chromatography separates molecules based on their size, where small molecules can fit into the pores of the stationary phase and take longer to elute, while large molecules cannot pass through the pores and elute first. This statement is reflected in options I, II, and IV. Option III suggests that small molecules can effectively pass through a larger volume, but this is not necessarily true for molecular exclusion chromatography, where molecules must pass through the pores. Option V is also true as molecular exclusion chromatography with a hydrophilic stationary phase and aqueous solvent is known as gel permeation chromatography. Therefore, the correct choice is C, which includes statements I, II, and IV.

Which Statements Are True for Molecular Exclusion Chromatography