____________________ is used to isolate a single compound or class of compounds from a complex mixture by passing the sample through a column where only one solute is bound.The remaining solutes pass through the column.The bound solute is eluted from the column by changing a condition such as________________________________________ to weaken its binding.
A)Adhesion chromatography;ratio of polar and nonpolar solvents
B)Binding chromatography;pH or ionic strength
C)Affinity chromatography;the polarity of the solvent
D)Adhesion chromatography;the temperature of the column
E)Affinity chromatography;pH or ionic strength

Question

Quizplus · Accepted Answer

The technique described in the question is Affinity Chromatography which uses a specific receptor molecule to bind to the target compound, and then the bound compound is eluted by changing the pH or ionic strength of the solvent. Therefore, choice E (Affinity chromatography; pH or ionic strength) is the correct answer.

____________________ Is Used to Isolate a Single Compound or Class