You want to amplify a region of yeast DNA using PCR so that this fragment can be cloned into a plasmid.Which of the following is needed for the PCR?
A)RNA polymerase
B)DNA ligase
C)DNA helicase
D)Taq polymerase

Question

Quizplus · Accepted Answer

Taq polymerase is needed for the PCR as it is a heat-stable DNA polymerase that can withstand the high temperatures required during the PCR process. RNA polymerase is used for transcription, not PCR. DNA ligase is used for joining two DNA fragments together. DNA helicase is used for unwinding the double-stranded DNA during replication, but not for PCR.

You Want to Amplify a Region of Yeast DNA Using